AOAC – RI #021201

 

AOAC – RI #021201

 

INTENDED USER

The IEH Listeria spp. and Listeria monocytogenes Test System (IEH Listeria Test) is designed for use by laboratory technicians with proper aseptic techniques needed to perform DNA amplification-based assays in a biosafety level 2 laboratory environment.

APPLICABILITY

The IEH Listeria Test allows rapid detection of Listeria sp. and specifically Listeria monocytogenes in foods such as raw meats, RTE products, leafy greens and environmental samples. Presumptive identification is completed within 24 hours.

It is recommended that test samples for which presumptive positive results are obtained be confirmed by subsequent application of confirmation procedures described in BAM (http://www.cfsan.fda.gov/~ebam/bam-10.html) or FSIS MLG http://www.fsis.usda.gov/PDF/MLG_8_05.pdf).

INTERPRETATION CRITERIA

The IEH Listeria Test simultaneously detects the presence (or absence) of Listeria spp. and Listeria monocytogenes. Specific fragments of the bacterial DNA, which are unique to the target organisms and not present in other bacteria, are amplified using DNA polymerase.

A positive result for either pathogen in food is an indication that it may be unsafe for consumption, and that appropriate protective steps should be taken.

 

Test Kit Reagents Provided in IEH Listeria Test

  • 96 PCR L4 Lysis Buffer Tubes
  • 96 PCR LG Lysis Buffer Tubes
  • 96 PCR LM Lysis Buffer Tubes
  • 288 PCR Reaction Tubes with Lyophilized Enzyme
  • Molecular Size Standards
  • Positve Control Reactions
  • IEH Listeria Enrichment Media
  • IEH Electrophoresis Running Buffer with 0.034 mg/L Ethidium Bromide
  • IEH Gel Buffer
  • Low EEO Agarose

 

Listeria monocytogenes IEH Test System

Listeria spp. IEH Test Kit

 

 

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