Gluten is a composite protein found in cereal grains such as wheat, barley, and rye. Adverse immune responses to ingested gluten are relatively common and can cause debilitating symptoms. Since there is no cure, affected individuals must adopt a strictly gluten-free diet. Accordingly, numerous countries require that “gluten-free” food labels meet certain criteria. To assist the food industry in establishing effective food safety practices, AllergenControl™ has developed a number of rapid test kits that can reliably detect gluten residues in foods and on surfaces and equipment.
This test kit is designed for use in the qualitative determination of gluten from wheat, barley, and rye residues obtained from sampling foods (solids, powders, and liquids) environmental surfaces. The test is intended for laboratory use only and should only be performed by trained personnel.
The AllergenControl™ Gluten Residue Lateral Flow Test features polyclonal antibodies directed against gliadin, hordein, and secalin configured in both sandwich and competitive format for hydrolyzed gluten residues. To operate the kit, the sample is first subjected to a short extraction step. The sample extract is then diluted and applied to the lateral flow device sample port, where it is allowed to migrate across the reagent zone thus enabling visualization of the Sandwich Test Line (T) and Competitive Test Line (0) results. In addition, the reagent zone includes a Procedural Control Line (C) to minimize the potential for false negative outcomes.
(Product code: PA-F14)
Limit of Detection*: 2 ppm gluten
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
(Product Code: PA-F27)
Limit of Detection*: 10 ppm gluten
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
(Product Code: PA-F20)
Limit of Detection*: 20 ppm gluten
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
(Product Code: PA-E14)
Limit of Detection*: 0.2 μg gluten/swab, 2 ppm gluten (rinse water)
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
(Product Code: PA-E27)
Limit of Detection*: 1.0 μg gluten/swab, 10 ppm gluten (rinse water)
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
(Product Code: PA-E20)
Limit of Detection*: 2.0 μg gluten/swab, 20 ppm gluten (rinse water)
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min
* matrix-free determination
For additional information or assistance with using this test kit, please contact Microbiologique at (206)525-0412.