Detection of Initial Reaction for:
- E. coli o157
- STEC
- Salmonella
AOAC – RI – #100701
Intended Use
The IEH E. coli O157, Stx-producing E. coli (STEC) with Intimin and Salmonella Test System (IEH Test System) is designed for use by laboratory technicians with proper aseptic techniques needed to perform DNA amplification-based assays in a biosafety level 2 laboratory environment.
Applicability
The IEH Test System (AOAC PTM 100701) provides a procedure for the preparation of test samples, and a protocol for the rapid detection of Escherichia coli O157, Shiga-toxin producing E. coli (STEC) with Intimin and Salmonella spp. in foods such as raw beef trim, raw ground beef, raw poultry, ready-to-eat (RTE) products (excluding STEC), and mixed leafy greens.
Test Kit Reagents Provided in IEH Test System
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96 PCR ES6 Buffer Tubes (store at or below -20±5ºC until use for up to 1 year)
- 288 PCR Reaction Tubes with Lyophilized Enzyme (store at room temperature for up to 6 months)
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288 PCR Reaction Tubes with Lyophilized Enzyme (store at room temperature for up to 6 months)
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288 DNA Lateral Flow Test Strips (ES6, EC7 and STP) (15-30ºC for up to 1 year)
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40 ml Lateral Flow Detection Buffer (Store at 15-30ºC for up to 1 year)
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Positive Control Reactions
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Enrichment Media (stable for up to six months after autoclaving).
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Sterile Deionized Water
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Bacto-peptone
E. Coli 0157 IEH Test System Part # PB100701O157
STEC IEH Test System Part # PB100701STEC
Salmonella IEH Test System Part # PB100701SAL
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