AOAC – RI – #100701

 

Detection of Initial Reaction for:

 

  • E. coli o157                    
  • STEC                                
  • Salmonella                     

 

AOAC – RI – #100701

 

 

Intended Use

The IEH E. coli O157, Stx-producing E. coli (STEC) with Intimin and Salmonella Test System (IEH Test System) is designed for use by laboratory technicians with proper aseptic techniques needed to perform DNA amplification-based assays in a biosafety level 2 laboratory environment.

 

Applicability

The IEH Test System (AOAC PTM 100701) provides a procedure for the preparation of test samples, and a protocol for the rapid detection of Escherichia coli O157, Shiga-toxin producing E. coli (STEC) with Intimin and Salmonella spp. in foods such as raw beef trim, raw ground beef, raw poultry, ready-to-eat (RTE) products (excluding STEC), and mixed leafy greens.

 

Test Kit Reagents Provided in IEH Test System

  • 96 PCR ES6 Buffer Tubes (store at or below -20±5ºC until use for up to 1 year)

  • 288 PCR Reaction Tubes with Lyophilized Enzyme (store at room temperature for up to 6 months)
  • 288 PCR Reaction Tubes with Lyophilized Enzyme (store at room temperature for up to 6 months)

  • 288 DNA Lateral Flow Test Strips (ES6, EC7 and STP) (15-30ºC for up to 1 year)

  • 40 ml Lateral Flow Detection Buffer (Store at 15-30ºC for up to 1 year)

  • Positive Control Reactions

  • Enrichment Media (stable for up to six months after autoclaving).

  • Sterile Deionized Water

  • Bacto-peptone

 

 

E. Coli 0157 IEH Test System                              Part # PB100701O157

STEC IEH Test System                                           Part # PB100701STEC

Salmonella IEH Test System                               Part # PB100701SAL

 

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