Mustard Microwell

Mustard, belonging to the cabbage genus Brassica, is one of the most commonly used spices and includes three common varieties: black, brown, and yellow mustard. Mustard may induce a range of allergic symptoms including severe anaphylactic reactions in sensitized individuals. Mustard is widely used as a flavoring agent, as well as for its texture enhancing and emulsification capacity in various food preparations, particularly with salad dressings and meats. Due to the increase prevalence of allergy to mustard, it is regulated in Canada and Europe as a declared food allergen.

Intended Use

The ELISA SYSTEMS® Mustard Residue Detection Kit™ is an enzyme-linked immunosorbent assay (ELISA) that may be used to screen food products for the presence of mustard residues introduced into foods via cross-contamination during manufacturing or direct contamination of raw ingredients. This assay is a rapid and reliable test that significantly reduces the time required to screen food products for the presence of mustard.

Assay Principles

The ELISA SYSTEMS® Mustard Residue Detection Kit™ is based on the sandwich enzyme-linked immunosorbent assay format. The food sample is first subjected to an extraction procedure and then tested by ELISA for the presence of mustard allergens. The results may be quantified by comparing the test results against results obtained from protein standards supplied in the kit.

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(Product code: EM52910)

Performance Characteristics

Range of Quantification: 0.1 – 10.0 ppm
Sample Preparation Time: ~15 min
Test period: ~35 min