Hazelnut Microwell

The hazelnut (Corylus avellana) belongs to a group of foods, commonly referred to as “tree nuts”. This group of foods is a significant source of allergens, and hazelnuts are a frequent cause of food allergy. Consequently, there is a need for a sensitive and reliable test for the detection of specific tree nut allergens in foods. Any such test must be able to detect a heat stable protein component, otherwise false negative results could occur with samples that have been highly heat treated.

A specific hazelnut protein was identified from high temperature treatments to be very heat stable and is used as the hazelnut protein indicator for the ELISA SYSTEMS® Hazelnut Residue Kit.

Intended Use

The ELISA SYSTEMS® Hazelnut Residue Detection Kit is an enzyme-linked immunosorbent assay (ELISA) that may be used to rapidly screen food products and environmental samples for the presence of hazelnut material.

Assay Principles

The ELISA SYSTEMS® Hazelnut Residue Detection Kit test is based on the sandwich enzyme-linked immunosorbent assay format. The food sample is first subjected to an extraction procedure and then tested by ELISA for the presence of hazelnut allergens. The assay detects multiple hazelnut proteins.  The results may be quantified by comparing the test results against results obtained from protein standards supplied in the kit.

kitimage

(Product code: EH52710)

Performance Characteristics

Range of Quantification: 0.5 – 5.0 ppm Hazelnut Protein
Sample Preparation Time: ~15 min
Test period: ~35 min