Casein Lateral Flow

Allergies to cow’s milk are one of the most common food allergies, affecting roughly 3% of the population. Consequently, numerous countries require that food labels clearly indicate milk content. Casein is the major protein in milk (80% of total milk proteins) and constitutes a major allergen of clinical importance. Casein is selectively added or present in certain foods. To assist the food industry in establishing effective food safety practices, AllergenControl™ has developed a rapid test kit that can reliably detect Casein residues in food and on surfaces and equipment.

Intended Use

This test kit is designed for use in the qualitative determination of casein residues obtained from sampling foods (solids, powders, and liquids) and surfaces. The test is intended for laboratory use only and should only be performed by trained personnel.

Assay Principles

The AllergenControl™ Casein Residue Lateral Flow Test features polyclonal antibodies directed against casein proteins configured in a sandwich format. To operate the kit, the sample is first subjected to a short extraction step. The sample extract is then directly applied to the lateral flow device sample port, where it is allowed to migrate across the reagent zone thus enabling visualization of the Sandwich Test Line (T) results. In addition, the reagent zone includes a Procedural Control Line (C) to minimize the potential for false negative outcomes.

Elisa Systems Lateral Flow Test Device

Food Sampling Test

(Product Code: PA-F12)

Performance Characteristics

Limit of Detection*: 1.0 μg protein/g or 1.0 ppm
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min

Environmental Surface Test

(Product Code: PA-E12)

Performance Characteristics

Limit of Detection: 0.1 μg protein/swab, 1.0 ppm protein (rinse water)
Sample Extraction Time: 1 min or 15 min
Test Operation Time: 15 min

* matrix-free determination